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Laboratory Technician II

WHAT YOU WILL ACHIEVE

This position will be responsible for executing quality control-related assays such as miSeq next-generation sequencing (NGS), Nanopore sequencing, and diagnostic restriction digests, as well as producing DNA preparations at various scales (high-throughput, small scale, large scale).

These contributions will support the plasmid & DNA production lab’s role in fulfilling Addgene’s mission of making high quality materials available to the scientific community.

POSITION REPORTS TO

Sr. DNA Services Manager

APPROXIMATE START DATE

ASAP

SALARY

$55,000-57,000 annually + our amazing benefits (see below)!

LOCATION & POLICY ON REMOTE WORK

This position will work out of our Watertown, Massachusetts Headquarters. Our beautiful office space has free, ample parking with a complimentary shuttle from Harvard Square and is on multiple bus lines, with the cost of public transit covered by Addgene.

The Role:

A Lab Technician II is responsible for executing the miSeq NGS and Nanopore sequencing pipelines and producing high yield DNA preps. Under the direction of the Sr. DNA Services Manager, the Lab Technician II will ensure on-time delivery of DNA-based samples and sequencing data to support the work of the other production teams.

To Succeed in this Role, You Will:

Execute upstream processing steps necessary for production, QC, and/or order fulfillment of DNA-based product requests. This includes small and large scale DNA prepping, QC techniques for DNA quantification, and NGS sequencing.
Prepare and load Illumina and Oxford Nanopore libraries on the miSeq or Nanopore machines, respectively. Troubleshoot issues that may arise and ensure successful completion of a sequencing run.
Conduct quality control of DNA samples including quantifying DNA concentrations and performing restriction digests of DNA samples prepared via low and high throughput pipelines
Troubleshoot issues that arise during lab processing and DNA preparation
Use Addgene’s computer software to process DNA-based products and inventoried items
Liaise with other lab technicians, Scientific Support, and QC teams for customer-related requests
Assist with process optimization and troubleshooting that may arise in collaboration with manager
Support lab operations in ensuring all lab consumables and reagents required for essential functions of the position are readily available for timely delivery of results. May include assisting with inventory management, solution preparation, and standard lab tasks.
Develop and maintain standard operating procedures (SOPs) within the team

Skills and Competencies

M.S. in a biological science discipline or a B.S./B.A. in biological sciences with 2 years related experience
Experience with NGS (eg Illumina or Oxford Nanopore) required
Demonstrated aseptic technique and working knowledge of general micro/molecular biology techniques required
Experimental lab experience (can be academic) and experimental design preferred
Experience with plasmid DNA purification preferred
Effective process management skills
Self-motivated, organized, able to work independently
Must be able to communicate effectively in English; strong written and oral communication skills required
Familiarity with general computer software, including Google Docs and web applications

Benefits

At Addgene, we prioritize our people with comprehensive benefits that support both your work and your life.

Full-time employees receive:

100% company-paid benefit package that includes health & dental (extending to family members), life, short-term, and long-term disability insurance.
Twenty days of PTO on top of our winter break closure and company holidays. ‌
A 403(b) retirement account with a company match of 4% after 2 years of employment. ‌
Annual professional development stipend
Annual lifestyle stipend
Fully paid public transportation for employees who work onsite four or more days per week. ‌
Onsite yoga classes & wellness reimbursement
Pet insurance
Annual financial planning one-on-ones with our plan administrator

The Organization:

Addgene is a self-sustaining, thriving nonprofit life sciences organization that was created to help scientists share DNA-based research materials called plasmids. ‌Our mission is to accelerate research and discovery by improving access to useful research materials and information. Since its founding in 2004, Addgene has grown and innovated in order to assist the research community by archiving and sharing useful DNA-based research materials and resources (such as plasmids, viral vectors, and recombinant antibodies) with scientists worldwide. Broad availability of these unique research materials promotes the advancement of research in a wide variety of disciplines, including cancer, heart disease, and neurodegenerative disorders, while increasing collaboration within the scientific community. Learn more here from one of our founders, Melina Fan.

As an “Addgenie”, you will work collaboratively with internal teams to help fulfill this mission, including scientists, software engineers and product developers, renowned customer support teams, scientific content creators, and business development professionals. Together, Addgenies create and enhance opportunities for research scientists to deposit their materials, find products and resources easily on our website, and receive unparalleled support in receiving and expertly using our materials in their own experiments.

Life at Addgene:

Addgene is a truly special place to work. We are an inclusive and welcoming group of people from many backgrounds and perspectives. We believe in flexibility, actively supporting professional development, and a strong work/life balance. We value sharing ideas and feedback with each other and the company. Learn more about Life at Addgene

We also believe it's important to make time for fun, whether it's eating popcorn at our outdoor movie night, sharing cute photos in the #pets slack channel, or sampling whiskeys at our end-of-year social hour. Bookworms, athletes, gamers, parents, foodies, and more will find community in Addgene's events and Slack channels.

Addgene works with a global scientific community in which the best innovations and discoveries come from diverse teams. We continuously learn from and celebrate a variety of perspectives and seek to create an environment where all people can feel heard, respected, and valued.